Comprehensive Guide to TCP1 Alpha Antibody (23c CTA-123)
The TCP1 alpha antibody, also known as 23c (CTA-123), plays a vital role in biological research and therapeutic applications. This monoclonal antibody targets the TCP1 protein, which is crucial for the proper functioning of chaperonin complexes involved in protein folding. In this guide, we will explore the various types, applications, and technical specifications of the TCP1 alpha antibody, providing you with an in-depth understanding of its significance in the scientific community.
Comparison of TCP1 Alpha Antibodies
| Type | Source | Application | Catalog Number | Supplier |
|---|---|---|---|---|
| TCP1 alpha Antibody (DyLight 405) | Rat Monoclonal | Western Blot, Immunofluorescence | NBP250328V | www.fishersci.com |
| TCP1 alpha Antibody (Alexa Fluor® 488) | Rat Monoclonal | Immunofluorescence | NBP250328V | www.novusbio.com |
| TCP1 alpha Antibody (BSA Free) | Rat Monoclonal | ELISA, Immunoprecipitation | N/A | www.bio-techne.com |
| Anti-TCP1 (23c) | Mouse Monoclonal | Western Blot, Flow Cytometry | N/A | ximbio.com |
What is TCP1 Alpha Antibody?
TCP1 alpha antibody (23c) is a monoclonal antibody that specifically recognizes the TCP1 protein, a component of the chaperonin complex that assists in the folding of newly synthesized proteins. This antibody is essential for researchers studying protein dynamics and cellular stress responses.
Applications of TCP1 Alpha Antibody
TCP1 alpha antibodies are utilized in various research applications, including:
1. Western Blotting
Western blotting is a widely used technique to detect specific proteins in a sample. The TCP1 alpha antibody provides high specificity and sensitivity, allowing for accurate protein quantification.
2. Immunofluorescence
In immunofluorescence assays, TCP1 alpha antibodies are employed to visualize the localization of TCP1 proteins within cells. This method is crucial for understanding cellular processes and interactions.
3. ELISA
Enzyme-linked immunosorbent assays (ELISA) leverage TCP1 alpha antibodies to quantify protein levels in complex mixtures, aiding in diagnostics and biochemical research.
4. Immunoprecipitation
TCP1 alpha antibodies can be used for immunoprecipitation, allowing for the isolation of TCP1 proteins from cell lysates for further analysis.
Technical Features of TCP1 Alpha Antibody
| Feature | Description |
|---|---|
| Host Species | Rat or Mouse |
| Isotype | IgG1 |
| Specificity | TCP1 Protein |
| Recommended Dilution | 1:200 to 1:1000 |
| Storage Conditions | -20°C to -80°C |
| Catalog Availability | NBP250328V (Novus Biologicals) |
Choosing the Right TCP1 Alpha Antibody
When selecting a TCP1 alpha antibody, consider the following factors:
1. Source Species
The source of the antibody (rat or mouse) can affect its performance in different assays. Rat monoclonal antibodies are commonly used for their specificity.
2. Application Suitability
Ensure the chosen antibody is validated for your specific application, such as Western blotting or immunofluorescence. Different suppliers like www.novusbio.com and www.bio-techne.com may provide antibodies suited for various applications.
3. Conjugation Options
Antibodies can be conjugated to different fluorophores or enzymes, such as Alexa Fluor® or DyLight, which enhance detection capabilities during assays.
4. Support and Documentation
Choose suppliers that provide comprehensive datasheets, validation studies, and customer support, ensuring you have access to all necessary information for successful experimentation.
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Conclusion
The TCP1 alpha antibody (23c CTA-123) is a powerful tool for biological research, offering various applications in protein analysis. Its specificity, versatility, and available forms make it a valuable asset in laboratories. With careful selection based on application, source, and technical specifications, researchers can leverage the TCP1 alpha antibody to gain insights into protein dynamics and cellular processes.
FAQ
What is TCP1 alpha antibody used for?
TCP1 alpha antibody is primarily used for detecting and studying the TCP1 protein in various applications such as Western blotting, immunofluorescence, ELISA, and immunoprecipitation.
How does TCP1 alpha antibody function?
This monoclonal antibody binds specifically to the TCP1 protein, allowing researchers to visualize or quantify the protein in cell samples, thereby aiding in the study of protein folding and cellular stress.
What are the storage conditions for TCP1 alpha antibody?
TCP1 alpha antibodies should be stored at -20°C to -80°C to maintain their stability and functionality over time.
Can TCP1 alpha antibody be used in human samples?
Yes, TCP1 alpha antibodies can be used in human-derived samples, but it is essential to confirm the antibody’s specificity and suitability for human tissues.
Where can I purchase TCP1 alpha antibody?
TCP1 alpha antibodies are available from various suppliers, including www.fishersci.com, www.novusbio.com, and www.bio-techne.com.
What is the recommended dilution for TCP1 alpha antibody?
The recommended dilution for TCP1 alpha antibody typically ranges from 1:200 to 1:1000, depending on the specific application.
Are there different types of TCP1 alpha antibodies?
Yes, there are various types of TCP1 alpha antibodies, including those conjugated to different fluorophores for specific applications like immunofluorescence.
What is the significance of TCP1 protein in cells?
TCP1 protein plays a critical role in the folding of newly synthesized proteins, which is essential for proper cellular function and response to stress.
What are the validation methods for TCP1 alpha antibody?
Validation methods may include Western blotting, immunofluorescence, and ELISA to ensure the antibody’s specificity and effectiveness in detecting TCP1 protein.
Can I use TCP1 alpha antibody for flow cytometry?
Yes, TCP1 alpha antibodies can be used for flow cytometry, but it is crucial to verify their performance in this specific application from the supplier.
